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首页 > 学术中心 > 学术ASAP > Electrospinning of a PMA-co-PAA/FP biopolymer nanofiber
 

Electrospinning of a PMA-co-PAA/FP biopolymer nanofiber

 
发布日期:2015-05-18     来源:永康乐业
 

Electrospinning of a PMA-co-PAA/FP biopolymer nanofiber: enhanced capability for immobilized horseradish peroxidase and its consequence forp-nitrophenol disposal.

Changlong Li,  Lei ZhouCuie Wang,   Xinhua Liu and  Kaiming Liao

RSC Adv., 2015,5, 41994-41998

DOI: 10.1039/C5RA05626H

上线时间:04 May 2015

Graphical abstract

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Abstract     

In this study, a novel poly(methyl acrylate-co-acrylic acid)/feather polypeptide (PMA-co-PAA/FP) composite nanofiber, which was prepared via the electrospinning technique, was employed as a support for scalable immobilization of horseradish peroxidase (HRP) for the first time. The resulting PMA-co-PAA/FP–HRP can function as an active and recyclable nanobiocatalyst for p-nitrophenol (PNP) disposal, which shows high stability (still retaining 80% of the initial activity after 35 days), and robust activity (even under a broad pH value of 5–9 and temperature range of 20–60 °C). Furthermore, the conversion capability of the PNP with the immobilized HRP was significantly higher than its unimmobilized counterpart, showing a conversion ratio up to 85%. After 5 repeated cycles, the conversion capacity of the immobilized HRP decreased slightly and could still retain 70% residual activity. These results indicated that the PMA-co-PAA/FP–HRP possessed high stability and excellent reusability, suggesting that the electrospun PMA-co-PAA/FP nanofibrous membrane might have potential applications in enzyme immobilization, particularly for those related to environmental protection. 静电纺丝

 

 

 

 
 
 

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